Enterococci have emerged among the leading causes of nosocomial infection.
With the goal of analyzing enterococcal genes differentially expressed in e
nvironments related to commensal or environmental colonization and infectio
n sites, we adapted and optimized a method more commonly used in the study
of eukaryotic gene expression, random arbitrarily primed PCR (RAP-PCR). The
RAP-PCR method was systematically optimized, allowing the technique to be
used in a highly reproducible manner with gram-positive bacterial RNA. In t
he present study, aerobiosis was chosen as a variable for the induction of
changes In gene expression by Enterococcus faecalis, Aerobically and anaero
bically induced genes were detected and identified to the sequence level, a
nd differential gene expression was confirmed by quantitative, specifically
primed RT-PCR, Differentially expressed genes included several sharing ide
ntity with those of other organisms related to oxygen metabolism, as well a
s hypothetical genes lacking identity to known genes.