EXPRESSION OF FIBROBLAST-GROWTH-FACTOR RECEPTORS IN PERIIMPLANTATION MOUSE EMBRYOS

Citation
Da. Rappolee et al., EXPRESSION OF FIBROBLAST-GROWTH-FACTOR RECEPTORS IN PERIIMPLANTATION MOUSE EMBRYOS, Molecular reproduction and development, 51(3), 1998, pp. 254-264
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Biology","Developmental Biology",Biology,"Cell Biology
ISSN journal
1040-452X
Volume
51
Issue
3
Year of publication
1998
Pages
254 - 264
Database
ISI
SICI code
1040-452X(1998)51:3<254:EOFRIP>2.0.ZU;2-Z
Abstract
FGF receptor (FGFR) function is essential during peri-implantation mou se development. To understand which receptors are functioning, we test ed for the expression of all four FGF receptors in peri-implantation b lastocysts. By RT-PCR, FGFR-3 and FGFR-4 were detected at high levels, FGFR-2 at lower levels, and FGFR-1 was detected at background levels compared to control tissues. Because FGFR-3 and FGFR-4 were detected a t the highest levels, we studied these in detail. Between 3.5 days aft er fertilization (E3.5) and E6.0, FGFR-4 mRNA was detected ubiquitousl y in the peri-implantation embryo, restricted to the inner cell mass ( ICM) and its derivatives and primitive endoderm by E6.0, and was not d etected at E6.5. FGFR-3 mRNA was detected ubiquitously in the periimpl antation embryo with a tendency towards extraembryonic cells. We teste d blastocyst outgrowths, a model for implantation, for FGFR-3 and FGFR -4 protein. FGFR-3 protein was detected in all cells early during the outgrowth. Later, FGFR-3 was detected in the extraembryonic endoderm a nd trophoblast giant cells (TGC), but not in the ICM. FGFR-4 protein w as detected in all cells of the implanting embryo, but was restricted to the ICM/primitive endoderm in later stage outgrowths. The distribut ion of the receptor proteins in the blastocyst outgrowths is similar t o the distribution of the mRNA detected by in situ hybridization of se ctions of embryos. The data suggest roles for FGFR-3 and FGFR-4 in per i-implantation development. (C) 1998 Wiley-Liss, Inc.