In order to understand the detail of genetic alternation on chromosome
22. we performed polymerase chain reaction analysis ol; microsatellit
e polymorphisms corresponding to 13 loci on chromosome 22. We examined
33 primary carcinoma tissues, 5 metastatic tissues and corresponding
normal tissues. We detected microsatellite instability (MI) in 14 (42.
4%) of 33 cases in this study. Loss of heterozygosity (LOH) was observ
ed in at least one locus in 24 (72.7%) of the 33 cases. Among the loci
examined, LOH was restricted to D22S274 on chromosome 22q13 in 11 40.
7%) of 27 informative cases. No significant correlation between histol
ogical differentiation and LOH was observed. These observations sugges
t that the incidence of LOH at chromosome 22q is high and is associate
d with the carcinogenesis of oral squamous cell carcinoma (SCC). The D
22S274 li:icus may play an important role in the development of oral S
CC and be the site harboring a putative tumor suppressor gene.