ALLELIC LOSS ON CHROMOSOME-22 IN ORAL-CANCER - POSSIBILITY OF THE EXISTENCE OF A TUMOR-SUPPRESSOR GENE ON 22Q13

Citation
A. Miyakawa et al., ALLELIC LOSS ON CHROMOSOME-22 IN ORAL-CANCER - POSSIBILITY OF THE EXISTENCE OF A TUMOR-SUPPRESSOR GENE ON 22Q13, International journal of oncology, 13(4), 1998, pp. 705-709
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
ISSN journal
1019-6439
Volume
13
Issue
4
Year of publication
1998
Pages
705 - 709
Database
ISI
SICI code
1019-6439(1998)13:4<705:ALOCIO>2.0.ZU;2-N
Abstract
In order to understand the detail of genetic alternation on chromosome 22. we performed polymerase chain reaction analysis ol; microsatellit e polymorphisms corresponding to 13 loci on chromosome 22. We examined 33 primary carcinoma tissues, 5 metastatic tissues and corresponding normal tissues. We detected microsatellite instability (MI) in 14 (42. 4%) of 33 cases in this study. Loss of heterozygosity (LOH) was observ ed in at least one locus in 24 (72.7%) of the 33 cases. Among the loci examined, LOH was restricted to D22S274 on chromosome 22q13 in 11 40. 7%) of 27 informative cases. No significant correlation between histol ogical differentiation and LOH was observed. These observations sugges t that the incidence of LOH at chromosome 22q is high and is associate d with the carcinogenesis of oral squamous cell carcinoma (SCC). The D 22S274 li:icus may play an important role in the development of oral S CC and be the site harboring a putative tumor suppressor gene.