E. Frahm et al., APPLICATION OF 23S RDNA-TARGETED OLIGONUCLEOTIDE PROBES SPECIFIC FOR ENTEROCOCCI TO WATER HYGIENE CONTROL, Systematic and applied microbiology, 21(3), 1998, pp. 450-453
Identification of enterococci species by hybridization with recently d
esigned species-specific and group-specific 23S rDNA-targeted oligonuc
leotide probes was superior to results obtained with a common biochemi
cal test panel. Considering these findings, a molecular biological pro
cedure for the detection of enterococci in water samples was developed
. A short enrichment is followed by an amplification step and a hybrid
ization reaction in microtiter plate format. The detection limit is ab
out 1 CFU/ml, and results are available within 26 h.