APPLICATION OF 23S RDNA-TARGETED OLIGONUCLEOTIDE PROBES SPECIFIC FOR ENTEROCOCCI TO WATER HYGIENE CONTROL

Citation
E. Frahm et al., APPLICATION OF 23S RDNA-TARGETED OLIGONUCLEOTIDE PROBES SPECIFIC FOR ENTEROCOCCI TO WATER HYGIENE CONTROL, Systematic and applied microbiology, 21(3), 1998, pp. 450-453
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
21
Issue
3
Year of publication
1998
Pages
450 - 453
Database
ISI
SICI code
0723-2020(1998)21:3<450:AO2ROP>2.0.ZU;2-0
Abstract
Identification of enterococci species by hybridization with recently d esigned species-specific and group-specific 23S rDNA-targeted oligonuc leotide probes was superior to results obtained with a common biochemi cal test panel. Considering these findings, a molecular biological pro cedure for the detection of enterococci in water samples was developed . A short enrichment is followed by an amplification step and a hybrid ization reaction in microtiter plate format. The detection limit is ab out 1 CFU/ml, and results are available within 26 h.