EFFECTS OF CARBON CONCENTRATION AND CARBON-TO-NITROGEN RATIO ON GROWTH, CONIDIATION, SPORE GERMINATION AND EFFICACY OF THE POTENTIAL BIOHERBICIDE COLLETOTRICHUM-COCCODES

Citation
X. Yu et al., EFFECTS OF CARBON CONCENTRATION AND CARBON-TO-NITROGEN RATIO ON GROWTH, CONIDIATION, SPORE GERMINATION AND EFFICACY OF THE POTENTIAL BIOHERBICIDE COLLETOTRICHUM-COCCODES, Journal of industrial microbiology & biotechnology, 20(6), 1998, pp. 333-338
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
20
Issue
6
Year of publication
1998
Pages
333 - 338
Database
ISI
SICI code
Abstract
The effect of carbon concentration and carbon-to-nitrogen ratio (C:N) as well as their interaction on Colletotrichum coccodes growth and spo rulation in submerged flask culture were evaluated. When C:N ratios we re held constant, both mycelial dry biomass and spore yield increased with increasing carbon concentration. The specific spore yields (spore yield g(-1) carbon), however, were not significantly different for th e same C:N ratio in most cases. The highest spore yields (1.3 x 10(8) spores per ml) were obtained from media containing 20 g per liter carb on with C:N ratios ranging from 5:1 to 10:1. When the C:N ratio was gr eater than 15:1, spore yields were significantly decreased with increa sing C:N ratios. High carbon concentration (20 g L-1) combined with hi gh C:N ratios (above 15:1) reduced both mycelial growth and sporulatio n, and increased spore matrix production. Spores produced in medium co ntaining 10 g L-1 carbon with C:N ratios from 10:1 to 15:1 had 90% ger mination on potato dextrose agar after 12 h and caused extensive shoot dry weight reduction on the target weed, velvetleaf. These results su ggest that C:N ratios from 10:1 to 15:1 are optimal for C. coccodes sp ore production.