HETEROLOGOUS EXPRESSION OF THE DESULFOVIBRIO-GIGAS [NIFE] HYDROGENASEIN DESULFOVIBRIO FRUCTOSOVORANS MR400

Citation
M. Rousset et al., HETEROLOGOUS EXPRESSION OF THE DESULFOVIBRIO-GIGAS [NIFE] HYDROGENASEIN DESULFOVIBRIO FRUCTOSOVORANS MR400, Journal of bacteriology, 180(18), 1998, pp. 4982-4986
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ISSN journal
0021-9193
Volume
180
Issue
18
Year of publication
1998
Pages
4982 - 4986
Database
ISI
SICI code
0021-9193(1998)180:18<4982:HEOTD[>2.0.ZU;2-Y
Abstract
The ability of Desulfovibrio fructosovorans MR400 Delta hynABC to expr ess the heterologous cloned [NiFe] hydrogenase of Desulfovibrio gigas was investigated. The [NiFe] hydrogenase operon from D. gigas, hynABCD , was cloned, sequenced, and introduced into D, fructosovorans MR400. A portion of the recombinant heterologous [NiFe] hydrogenase was total ly matured, exhibiting catalytic and spectroscopic properties identica l to those of the native D. gigas protein. A chimeric operon containin g hynAB from D. gigas and hynC from D. fructosovorans placed under the control of the D. fructosovorans hynAp promoter was constructed and e xpressed in D. fructosovorans MR400. Under these conditions, the same level of activity was obtained as with the D, gigas hydrogenase operon .