THE STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASE AS MODELED BY HOMOLOGY AND VALIDATED BY SITE-DIRECTED MUTAGENESIS

Citation
S. Pascarella et al., THE STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASE AS MODELED BY HOMOLOGY AND VALIDATED BY SITE-DIRECTED MUTAGENESIS, Protein science, 7(9), 1998, pp. 1976-1982
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
0961-8368
Volume
7
Issue
9
Year of publication
1998
Pages
1976 - 1982
Database
ISI
SICI code
0961-8368(1998)7:9<1976:TSOSHA>2.0.ZU;2-0
Abstract
We describe a model for the three-dimensional structure of E. coli ser ine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are kno wn. The model suggests that certain amino acid residues at the putativ e active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the propertie s of a number of site-directed mutants. New active site features are a lso proposed for further experimental testing.