DETECTION OF CANCER-CELLS IN THE BLOOD BY FACS SORTING OF CD45(-) CELLS

Citation
J. Baran et al., DETECTION OF CANCER-CELLS IN THE BLOOD BY FACS SORTING OF CD45(-) CELLS, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 1(3), 1998, pp. 573-578
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
1107-3756
Volume
1
Issue
3
Year of publication
1998
Pages
573 - 578
Database
ISI
SICI code
1107-3756(1998)1:3<573:DOCITB>2.0.ZU;2-C
Abstract
We describe a simple and sensitive method for detection of low number of cancer cells in the blood. The method is based on FAGS sorting of l eukocytes labelled with anti-CD45 monoclonal antibody and examining CD 45- cells by conventional cytology and immunostaining for cytokeratin 18. In a model study, cancer cells seeded at the frequency of 1 per 10 (6) and 1 per 10(7) leukocytes were detected in CD45-population. Sensi tivity of this method was comparable to reverse transcription polymera se chain reaction (RT-PCR) used for detection of cancer cells expressi ng CD44 variants-mRNA. In a pilot study, cancer cells were also isolat ed from the blood of some patients with locally advanced gastric cance r. This method may be useful for detection of circulating tumour cells in cancer patients.