NEURAL PRECURSOR CELL CHAIN MIGRATION AND DIVISION ARE REGULATED THROUGH DIFFERENT BETA-1 INTEGRINS

Citation
Ts. Jacques et al., NEURAL PRECURSOR CELL CHAIN MIGRATION AND DIVISION ARE REGULATED THROUGH DIFFERENT BETA-1 INTEGRINS, Development, 125(16), 1998, pp. 3167-3177
Citations number
64
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Developmental Biology
Journal title
ISSN journal
0950-1991
Volume
125
Issue
16
Year of publication
1998
Pages
3167 - 3177
Database
ISI
SICI code
0950-1991(1998)125:16<3167:NPCCMA>2.0.ZU;2-N
Abstract
Proliferation and tangential migration of neural precursor cells are e ssential determinants of CNS development. We Rave established cell cul ture models of both these processes using neural precursor cells grown as neurospheres. The pattern of migration that we observe in these ce lls is homotypic and occurs in the absence of a glial or neuronal scaf fold, and is therefore equivalent to that previously described as chai n migration. To determine the role of integrins in proliferation and m igration, we have analysed the expression pattern of integrins on neur osphere cells and then performed blocking peptide and antibody experim ents. Neurosphere cells express five major integrins, alpha 5 beta 1, alpha 6A beta 1, alpha v beta 1, alpha v beta 5 and alpha v beta 8 and , in addition, express low levels of alpha 6B beta 1. Chain migration is inhibited by blocking the alpha 6 beta 1 integrin, Proliferation, b y contrast, is inhibited by blocking the other beta 1 integrins, alpha v beta 1 and alpha 5 beta 1, These results show that integrins are im portant regulators of neural precursor cell behaviour, with distinct b eta 1 integrins regulating proliferation and migration. They also demo nstrate a novel role for the alpha 6 beta 1 integrin in the cell-cell interactions underlying homotypic chain migration.