PHYLOGENETIC POSITION AND CODON USAGE OF 2 CENTRIN GENES FROM THE RUMEN CILIATE PROTOZOAN, ENTODINIUM-CAUDATUM

Citation
Scp. Eschenlauer et al., PHYLOGENETIC POSITION AND CODON USAGE OF 2 CENTRIN GENES FROM THE RUMEN CILIATE PROTOZOAN, ENTODINIUM-CAUDATUM, FEMS microbiology letters, 166(1), 1998, pp. 147-154
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ISSN journal
0378-1097
Volume
166
Issue
1
Year of publication
1998
Pages
147 - 154
Database
ISI
SICI code
0378-1097(1998)166:1<147:PPACUO>2.0.ZU;2-X
Abstract
A lambda phage cDNA expression library was constructed from washed sus pensions of the rumen ciliate protozoan, Endodinium caudatum, which ha d been maintained in an isolated, monofaunated sheep. The library was screened using an anti-E. caudatum antiserum raised in rabbits against sonically disrupted protozoa. DNA sequences for two centrins or caltr actins, a subfamily of the EF-hand Ca2+-modulaled proteins which are c losely related, highly conserved cytoskelelal proteins, were identifie d and characterised. Their phylogenetic position was established relat ive to other centrin gene sequences. The two proteins showed homology to Paramecium tetraurelia centrins: 50 and 52% identities and 71 and 7 5% similarities in the protein sequence, over 99 and 110 amino acids f ragments. Codon usage and indices revealed that E. caudatum follows un iversal codon usage, but with a restricted number of codons, and has a low G+C content. (C) 1998 Federation of European Microbiological Soci eties. Published by Elsevier Science B.V. All rights reserved.