NONINVASIVE, IN-UTERO IMAGING OF MOUSE EMBRYONIC HEART DEVELOPMENT WITH 40-MHZ ECHOCARDIOGRAPHY

Citation
S. Srinivasan et al., NONINVASIVE, IN-UTERO IMAGING OF MOUSE EMBRYONIC HEART DEVELOPMENT WITH 40-MHZ ECHOCARDIOGRAPHY, Circulation, 98(9), 1998, pp. 912-918
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Peripheal Vascular Diseas",Hematology,"Cardiac & Cardiovascular System
Journal title
ISSN journal
0009-7322
Volume
98
Issue
9
Year of publication
1998
Pages
912 - 918
Database
ISI
SICI code
0009-7322(1998)98:9<912:NIIOME>2.0.ZU;2-Q
Abstract
Background-The increasing number of transgenic and targeted mutant mic e with embryonic cardiac defects has resulted in the need for noninvas ive techniques to examine cardiac structure and function in early mous e embryos. We report the first use of a novel 40-MHz ultrasound imagin g system in the study of mouse cardiac development in utero, Methods a nd Results-Transabdominal scans of mouse embryos staged between 8.5 an d 13.5 days of gestation (E8.5 to E13.5) were obtained in anesthetized mice. Atrial and ventricular contractions could be discerned from E9. 5, and changes in cardiac morphology were observed from E9.5 to E13.5, Hyperechoic streaming patterns delineated flow through the umbilical, vitelline, and other major blood vessels. Diastolic and systolic vent ricular areas were determined by planimetry of the epicardial borders, and fractional area change was measured as an index of contractile fu nction. Significant increases in ventricular size were documented at e ach stage between E10.5 and E13.5, and the ability to perform serial i maging studies over 3 days of embryonic development is described. Fina lly, the detection of vascular cell adhesion molecule 1 (VCAM-1) homoz ygous null mutant embryos demonstrates the first example of noninvasiv e, in utero analysis of cardiac structure and function in a targeted m ouse mutant, Conclusions-We used 40-MHz echocardiography to identify k ey elements of the early mouse embryonic cardiovascular system and for noninvasive dimensional analysis of developing cardiac ventricles. Th e ability to perform serial measurements and to detect mutant embryos with cardiac defects highlights the usefulness of the technique for in vestigating normal and abnormal cardiovascular development.