MUTATION ANALYSIS OF THE WT1 GENE IN MYELODYSPLASTIC SYNDROMES

Citation
N. Hosoya et al., MUTATION ANALYSIS OF THE WT1 GENE IN MYELODYSPLASTIC SYNDROMES, Japanese journal of cancer research, 89(8), 1998, pp. 821-824
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
ISSN journal
0910-5050
Volume
89
Issue
8
Year of publication
1998
Pages
821 - 824
Database
ISI
SICI code
0910-5050(1998)89:8<821:MAOTWG>2.0.ZU;2-H
Abstract
The WT1 tumor suppressor gene was examined for mutations in a panel of 44 patients with myelodysplastic syndromes (MDS) including acute myel ogenous leukemias (AML) secondary to MDS, using polymerase chain react ion single strand conformation polymorphism (PCR-SSCP) analysis and se quencing analysis. A WT1 mutation was detected in one out of 17 cases of AML secondary to MDS. This mutation exists upstream of the zinc fin ger region and is predicted to produce a truncated WT1 protein lacking the zinc finger region, No mutations were detected in 27 MDS patients who had not progressed to AML. This is the first report of analysis f or WT1 mutations in a large number of MDS patients, suggesting that WT 1 mutations are uncommon in MDS, Abnormalities in this gene may, howev er, contribute to a small proportion of cases showing progression from MDS into AML.