ENDOTHELIUM-DEPENDENT POTENTIATION OF PROSTAGLANDIN F-2-ALPHA-INDUCEDCONTRACTIONS BY (+ -)-[6]-GINGEROL IS INHIBITED BY CYCLOOXYGENASE-INHIBITORS BUT NOT LIPOXYGENASE-INHIBITORS IN MOUSE MESENTERIC VEINS/

Citation
Y. Hata et al., ENDOTHELIUM-DEPENDENT POTENTIATION OF PROSTAGLANDIN F-2-ALPHA-INDUCEDCONTRACTIONS BY (+ -)-[6]-GINGEROL IS INHIBITED BY CYCLOOXYGENASE-INHIBITORS BUT NOT LIPOXYGENASE-INHIBITORS IN MOUSE MESENTERIC VEINS/, Biological & pharmaceutical bulletin, 21(8), 1998, pp. 792-794
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy
ISSN journal
0918-6158
Volume
21
Issue
8
Year of publication
1998
Pages
792 - 794
Database
ISI
SICI code
0918-6158(1998)21:8<792:EPOPF>2.0.ZU;2-F
Abstract
The mechanism of potentiation of prostaglandin (PG) F-2 alpha-induced contraction of mouse mesenteric veins by (+/-)-[6]-gingerol was invest igated in vitro. (+/-)-[6]-Gingerol (0.3 mM) potentiated the maximal c ontraction response elicited by PGF(2 alpha) (0.28 mM) in the presence of intact vascular endothelium, but not in its absence (de-endothelia lized preparations). The potentiating effect was completely inhibited by cyclooxygenase inhibitors (0.2 mM aspirin and 0.2 mM indomethacin) and partly by calcium antagonists (2 mu m verapamil, 8 nM nitrendipine and 1 mu M ryanodine), but not inhibited by nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor and ONO-3708, a thromboxane (TX) A(2 ) antagonist. The potentiation by (+/-)-[6]-gingerol is also observed in mesenteric veins of streptozotocin-diabetic mice where the enhancem ent of PGF(2 alpha)-induced contraction is caused mainly by activation of lipoxygenase. The potentiation of PGF(2 alpha)-dinduced contractio n by (+/-)-[6]-gingerol may be caused by a cyclooxygenase-dependent re lease of vasoconstrictors, other than PGF(2 alpha) and TXA(2), or by i nhibiting vasorelaxants released from endothelial cells of mouse mesen teric veins.