FREQUENCY OF RECIPROCAL TRANSLOCATIONS AND DICENTRICS INDUCED IN HUMAN BLOOD-LYMPHOCYTES BY X-IRRADIATION AS DETERMINED BY FLUORESCENCE IN-SITU HYBRIDIZATION

Citation
M. Nakano et al., FREQUENCY OF RECIPROCAL TRANSLOCATIONS AND DICENTRICS INDUCED IN HUMAN BLOOD-LYMPHOCYTES BY X-IRRADIATION AS DETERMINED BY FLUORESCENCE IN-SITU HYBRIDIZATION, International journal of radiation biology, 64(5), 1993, pp. 565-569
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
ISSN journal
0955-3002
Volume
64
Issue
5
Year of publication
1993
Pages
565 - 569
Database
ISI
SICI code
0955-3002(1993)64:5<565:FORTAD>2.0.ZU;2-Q
Abstract
This study was designed to test the scoring efficiency of reciprocal t ranslocations and dicentrics induced by X-irradiation in vitro using t he fluorescence in situ hybridization (FISH) technique. An excess was found in the frequencies of reciprocal translocations relative to thos e of dicentrics by measurement with FISH at the first cell division af ter irradiation (translocation:dicentric almost-equal-to 60:40). Howev er, when the same metaphases were also evaluated sequentially by a con ventional staining method, the ratio of about 50:50 was restored. This was due in part to misclassification of certain dicentrics as. recipr ocal translocations by the FISH technique.