EXPRESSION OF AN ALLOZYME OF PRORENIN-CONVERTING ENZYME IN THE SUBMANDIBULAR-GLAND OF DBA 2N MICE/

Citation
K. Hosoi et al., EXPRESSION OF AN ALLOZYME OF PRORENIN-CONVERTING ENZYME IN THE SUBMANDIBULAR-GLAND OF DBA 2N MICE/, Journal of Biochemistry, 124(2), 1998, pp. 368-376
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
0021-924X
Volume
124
Issue
2
Year of publication
1998
Pages
368 - 376
Database
ISI
SICI code
0021-924X(1998)124:2<368:EOAAOP>2.0.ZU;2-Z
Abstract
A protein product of the tissue kallikrein gene family was isolated fr om the submandibular gland of DBA/2N mice. Amino acid sequencing showe d this protein to be highly homologous to two tissue kallikreins, mK13 and mK26, also known as prorenin-converting enzymes PRECE and PRECE-2 , respectively. The cDNA corresponding to the present enzyme was clone d, and its complete nucleotide sequence was determined. The cloned cDN A was different in 6 and 12 bases out of 783 nucleotides from those of mKlk-13 and mKlk-26 cDNAs, respectively, the homologies being 99.2 an d 98.5% (nucleotide), or 98.3 and 96.2% (amino acid). Upon incubation with either bovine kininogens or mouse Ren 2 prorenin, this tissue kal likrein generated bradykinin and renin, respectively, as judged by Wes tern blotting and protein sequence analysis. Isoelectric focusing anal ysis of the submandibular gland tissue kallikreins suggested that the present enzyme was not expressed in CD-1 or ICR mice and that no mK13 protein was present in DBA/2N mice. These data suggest that the enzyme is an allozyme of mK13, a prorenin-converting enzyme highly expressed in the submandibular glared of DBA/2N mice. The mKlk-13 gene in mice is therefore suggested to be polymorphic, having at least two allelic forms with a high sequence homology. The designation mK13(b) and mKlk0 13(b) for the protein and gene of this tissue kallikrein is proposed.