PROTEASE-CATALYZED SYNTHESIS OF PRECURSOR DIPEPTIDES OF RGD WITH REVERSE MICELLES

Citation
Yx. Chen et al., PROTEASE-CATALYZED SYNTHESIS OF PRECURSOR DIPEPTIDES OF RGD WITH REVERSE MICELLES, Enzyme and microbial technology, 23(3-4), 1998, pp. 243-248
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
0141-0229
Volume
23
Issue
3-4
Year of publication
1998
Pages
243 - 248
Database
ISI
SICI code
0141-0229(1998)23:3-4<243:PSOPDO>2.0.ZU;2-O
Abstract
The precursor dipeptides of RGD, Boc-Arg-Gly-OEt and Ac-Gly-Asp-diOMe, were synthesized in three reverse micellar systems using different pr oteases. The reaction conditions for the Boc-Arg-Gly-OEt synthesis cat alyzed by alcalase and for Ac-Gly-Asp-diOMe synthesis catalyzed by try psin in AOT/isooctane reverse micelles were optimized by examining the effects of several factors including water content, temperature, pH, reaction time, and the enzymes on the dipeptide yields. The best yield s for alcalase-catalyzed synthesis of Boc-Arg-Gly-OEt and trypsin-cata lyzed synthesis of Ac-Gly-Asp-diOMe were 62.7% and 78.8%, respectively . Two other reverse micelle systems, Triton/ethyl acetate and HTAB/hep tane/hexanol were also tested for the syntheses of the same dipeptides . (C) 1998 Elsevier Science Inc.