DETERMINATION OF SULFONAMIDES WITH FLUORESCENT INCLUSION COMPLEX OF CARBONIC-ANHYDRASE AND DANSYLAMIDE

Authors
Citation
Hn. Li et Yx. Ci, DETERMINATION OF SULFONAMIDES WITH FLUORESCENT INCLUSION COMPLEX OF CARBONIC-ANHYDRASE AND DANSYLAMIDE, Chinese Science Bulletin, 43(13), 1998, pp. 1085-1090
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
1001-6538
Volume
43
Issue
13
Year of publication
1998
Pages
1085 - 1090
Database
ISI
SICI code
1001-6538(1998)43:13<1085:DOSWFI>2.0.ZU;2-U
Abstract
A new fluorimetric method for determination of sulfonamides was descri bed based on the formation of a fluorescent inclusion complex of carbo nic anhydrase(CA) with dansylamide (DNSA). The binding of DNSA to CA r esulted in an enhancement in the fluorescence emission at 460 nm with excitation at 280 nm. Dissociation constants were determined for the c arbonic anhydrase-sulfonamide complexes. Linear calibration graphs of sulfonamides were obtained within a concentration range of 0-0.058 mu g/mL for DNSA; 0-0.344 mu g/mL for sulfanilamide (SAN) and 0-0.286 mu g/mL for P-toluene-sulfonamide (PTSN). The relative standard deviation s were within 1.8%-4.2%. Limits of detection for DNSA, SAN and PTSN we re 0.84, 19.5 and 6.1 ng/mL, respectively. The method was applied to t he determination of sulfonamides in cow milk at ng/mL level.