MOLECULAR CHARACTERIZATION OF A PANEL OF HUMAN OVARIAN-CARCINOMA XENOGRAFTS

Citation
Am. Codegoni et al., MOLECULAR CHARACTERIZATION OF A PANEL OF HUMAN OVARIAN-CARCINOMA XENOGRAFTS, European journal of cancer, 34(9), 1998, pp. 1432-1438
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
ISSN journal
0959-8049
Volume
34
Issue
9
Year of publication
1998
Pages
1432 - 1438
Database
ISI
SICI code
0959-8049(1998)34:9<1432:MCOAPO>2.0.ZU;2-8
Abstract
In a panel of 16 human ovarian tumours transplanted in nude mice, the expression of genes involved in cell cycle regulation and in response to drug treatment were characterised. In the 16 tumours analysed we co uld not detect overexpression of Erb-B2 oncogene while expression of M DR1 mRNA was not detected in 11/15 samples and was low in 4/15 tumours . Only three tumours had mutations in the p53 gene exons 5-8 and one o f these mutations did not result in any amino acid alteration. The lev els of mRNA for cyclins A, D1 and E were heterogeneous with some tumou rs expressing high levels and others not expressing them at all. The s ame was found for the cyclin dependent kinases (CDK) CDK2 and CDK4 and for CDK inhibitors P21/WAF1, p27/KIP1 and p16/CDKN2. Two genes belong ing to the nucleotide excision repair, ERCC1 and ERCC3 were detectable in all the samples examined, as were the genes MGMT and MAG, also inv olved in DNA repair. The data indicate a heterogeneity in the expressi on of genes considered to be involved in the cellular responses to cyt otoxic drug treatment and indicate the possibility of using these tumo ur models to test specifically molecules with a defined mechanism of a ction. (C) 1998 Elsevier Science Ltd. All rights reserved.