N. Yoshida et al., HEAVY-METAL PARTICLE RESISTANCE IN THIOBACILLUS-INTERMEDIUS 13-1 ISOLATED FROM CORRODED CONCRETE, Journal of fermentation and bioengineering, 85(6), 1998, pp. 630-633
The effect of exposure to heavy metal particles on the growth and surv
ival of bacterial cells was investigated. Thiobacillus intermedius 13-
1, Escherichia coli JM109, and Agrobacterium radiobacter IFO12665b1 we
re cultured on LB solid medium or in 5 mi of liquid medium containing
0.03 or 0.1g respectively of the heavy metals, aluminum, cadmium, iron
, lead, molybdenum, nickel, and zinc. Cadmium, nickel and zinc strongl
y inhibited cell growth in the three strains. In contrast, the bacteri
al cells were not inhibited by aluminum, iron, or lead in the solid or
liquid medium. When these bacteria were exposed to heavy metals by vi
gorous shaking for 10 min, lead and molybdenum, but not nickel and zin
c, were markedly toxic to the bacterial cells. Different reaction were
thus observed under low-level, long-term and high-level, short-term e
xposure conditions. T. intermedius showed more resistance to zinc and
nickel, and was more sensitive to molybdenum exposure, than E. coli an
d A. radiobacter.