USE OF MONOCLONAL-ANTIBODIES TO IDENTIFY PHOSPHOLIPASE-C AS THE ENTEROTOXIC FACTOR OF THE BIFUNCTIONAL HEMOLYSIN-PHOSPHOLIPASE-C MOLECULE OF VIBRIO-CHOLERAE-O139

Citation
S. Pal et al., USE OF MONOCLONAL-ANTIBODIES TO IDENTIFY PHOSPHOLIPASE-C AS THE ENTEROTOXIC FACTOR OF THE BIFUNCTIONAL HEMOLYSIN-PHOSPHOLIPASE-C MOLECULE OF VIBRIO-CHOLERAE-O139, Infection and immunity, 66(8), 1998, pp. 3974-3977
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
ISSN journal
0019-9567
Volume
66
Issue
8
Year of publication
1998
Pages
3974 - 3977
Database
ISI
SICI code
0019-9567(1998)66:8<3974:UOMTIP>2.0.ZU;2-2
Abstract
Two hybrid clones producing monoclonal antibodies (MAbs) raised agains t the purified enterotoxic hemolysin-phospholipase C (HlyPC) bifunctio nal molecule of a Vibrio cholerae O139 strain were used to study its e nterotoxicity in relation to its hemolytic and enzymatic activities. F ab fragments of MAbs from ascites produced by the two hybrids neutrali zed the hemolytic activity of HlyPC, leaving the enzymatic activity un affected. In ligated rabbit ileal loop and infant mouse intestine, the Fab fragments of the MAbs were not able to neutralize the enterotoxic ity of HlyPC, suggesting that PC rather than Hly is the enterotoxic mo iety of the molecule, The enterotoxicity of the purified PC molecule i solated from an Hly(-) spontaneous mutant of the HlyPC-producing paren t strain further confirms this contention, The Hly molecule isolated f rom a PC-mutant was not diarrheagenic.