MONITORING FOR INDUCED HERITABLE MUTATIONS IN NATURAL-POPULATIONS - APPLICATION OF MINISATELLITE DNA SCREENING

Authors
Citation
C. Yauk, MONITORING FOR INDUCED HERITABLE MUTATIONS IN NATURAL-POPULATIONS - APPLICATION OF MINISATELLITE DNA SCREENING, Mutation research. Reviews in mutation research, 411(1), 1998, pp. 1-10
Citations number
65
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Genetics & Heredity",Toxicology,"Biothechnology & Applied Migrobiology
ISSN journal
1383-5742
Volume
411
Issue
1
Year of publication
1998
Pages
1 - 10
Database
ISI
SICI code
1383-5742(1998)411:1<1:MFIHMI>2.0.ZU;2-P
Abstract
The need to understand the role that anthropogenic chemicals play in g enerating germline mutations is critical, both from an ecological and a human health perspective. Exposure to complex mixtures of urban and industrial chemicals is widespread and we have little understanding of the long-term implications to populations and gene pools. It has rece ntly been suggested that minisatellite DNA mutations may be sensitive biomarkers for induced heritable mutations in populations exposed to r adioactive and non-radioactive contamination in their environments. Mi nisatellite loci are attractive targets for mutational analyses becaus e they undergo a rate of mutation much greater than unique sequence DN A and with DNA fingerprinting many loci can be scanned simultaneously. As a result, the technique is statistically powerful requiring relati vely small sample sizes (compared to other in situ mutation assays) an d is reasonably cost and time efficient. This paper will review the ap plication of minisatellite mutation screening to the field of genetic toxicology. (C) 1998 Elsevier Science B.V. All rights reserved.