QUANTITATIVE-DETERMINATION OF AROMATIC-AMINO-ACIDS AND RELATED-COMPOUNDS IN RUMEN FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Citation
Ri. Khan et al., QUANTITATIVE-DETERMINATION OF AROMATIC-AMINO-ACIDS AND RELATED-COMPOUNDS IN RUMEN FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 710(1-2), 1998, pp. 17-25
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
1387-2273 → ACNP
Volume
710
Issue
1-2
Year of publication
1998
Pages
17 - 25
Database
ISI
SICI code
0378-4347(1998)710:1-2<17:QOAAR>2.0.ZU;2-Q
Abstract
A rapid method for the quantitative determination of tyrosine (Tyr), p henylalanine (Phe), p-hydroxybenzoic acid (HBA), p-hydroxyphenylacetic acid (HPA), benzoic acid (BZA), p-hydroxyphenylpyruvic acid (HPY), ph enylacetic acid (PAA), phenyllactic acid (PLA), tryptophan (Trp), indo leacetic acid (IAA), phenylpyruvic acid (PPY), phenylpropionic acid (P PA) and cinnamic acid (CNA) in goat rumen fluid was established by hig h-performance liquid chromatography (HPLC). The mobile phase used for isocratic elution was 50 mM sodium phosphate buffer (pH 6.5)-methanol (97:3, v/v). The flow-rate was 1.0 ml/min; column temperature 40 degre es C and compounds were monitored at 215 nm with a UV absorbance detec tor after injection of 10 mu l Of filtered rumen fluid. Analysis was c ompleted within 40 min. The minimum detectable limits of quantificatio n (mu M) of these compounds were Tyr, 2; Phe, 3; HBA, 1; HPA, 2; BZA, 2; HPY, 8; PAA, 3; PLA, 4; Trp, 2; IAA, 2; PPY, 15; PPA, 8 and CNA, 4. Detectable levels of Tyr, Phe, HPA, BZA, HPY, PAA, PLA, Trp and PPA w ere found in the deproteinized rumen fluid of goat fed a haycube and c oncentrate mixture. PAA was the predominant compound before and after feeding. The concentrations of HPA, BZA, PAA, PLA and PPA in the goat rumen fluid increased after feeding, while the concentration of Tyr de creased. Phe, HPY and Trp were minor components at all times. PPY, IAA and CNA were not detected and HBA was not completely resolved in the goat rumen fluid. (C) 1998 Elsevier Science B.V. All rights reserved.