EPITHELIAL VS MESENCHYMAL CONTRIBUTION TO THE EXTRACELLULAR-MATRIX INTHE HUMAN INTESTINE

Citation
N. Perreault et al., EPITHELIAL VS MESENCHYMAL CONTRIBUTION TO THE EXTRACELLULAR-MATRIX INTHE HUMAN INTESTINE, Biochemical and biophysical research communications (Print), 248(1), 1998, pp. 121-126
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006-291X
Volume
248
Issue
1
Year of publication
1998
Pages
121 - 126
Database
ISI
SICI code
0006-291X(1998)248:1<121:EVMCTT>2.0.ZU;2-H
Abstract
The basement membrane (BM) underlying the epithelium of the intestine is generally believed to be of both epithelial and mesenchymal origin but the exact contribution of each tissue has not been directly examin ed in the human. In this study, we have used a newly described procedu re to dissociate the human intestine into pure epithelial and correspo nding mesenchymal fractions. Northern blot and RT-PCR analyses of the fractions for the presence of transcripts encoding extracellular matri x molecules revealed that the epithelium produces the formal BM molecu les such as the alpha 1, alpha 2, and beta 1 chains of laminin-1 and l aminin-2 and the alpha 5(IV) and alpha 6(IV) chains of collagen as wel l as fibronectin, a BM-associated molecule. Interestingly, the alpha 1 (IV) chain of collagen, which associates with the alpha 2(IV) chain to form the main BM collagen network, as well as tenascin-C and decorin, two BM-associated molecules, was found to be exclusively of mesenchym al origin. Taken together, these data support the concept that in the human, as in experimental animals, the intestinal BM is composed of co mponents produced from both the epithelium and the mesenchyme. (C) 199 8 Academic Press.