DOWN-REGULATION OF TELOMERASE ACTIVITY IS AN EARLY EVENT OF CELLULAR-DIFFERENTIATION WITHOUT APPARENT TELOMERIC DNA CHANGE

Citation
O. Yamada et al., DOWN-REGULATION OF TELOMERASE ACTIVITY IS AN EARLY EVENT OF CELLULAR-DIFFERENTIATION WITHOUT APPARENT TELOMERIC DNA CHANGE, Leukemia research, 22(8), 1998, pp. 711-717
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,Hematology
Journal title
ISSN journal
0145-2126
Volume
22
Issue
8
Year of publication
1998
Pages
711 - 717
Database
ISI
SICI code
0145-2126(1998)22:8<711:DOTAIA>2.0.ZU;2-3
Abstract
With the use of three different hematopoietic cell lineages, the downr egulation of telomerase activity was found to be a general response to the induction of differentiation. The decrease in telomerase activity occurred as early as 24 h when HL-60 and K562 cells were cultured in the presence of 1 alpha, 25 dihydroxyvitamin D3 (VD3), all-trans-retin oic acid (ATRA) and hemin, and completely disappeared after 3 days. On the other hand, MEG-OI cells showed a marked inhibition of telomerase activity after 6 days of culture with 12-0-tetradecanoylphorbal 13-ac etate (TPA). The analysis of telomeric DNA in the HL-60 cells and K562 cells demonstrated no detectable loss of telomeric DNA with cellular differentiation, with a loss of telomerase activity. The repression of telomerase is a common molecular event during leukemic cell different iation. (C) 1998 Elsevier Science Ltd. All rights reserved.