PRODUCTION OF D-[1-C-13]GLUCOSE FROM [C-13]FORMALDEHYDE AND D-RIBOSE-5-PHOSPHATE USING AN ENZYMATIC SYSTEM

Citation
H. Maeda et al., PRODUCTION OF D-[1-C-13]GLUCOSE FROM [C-13]FORMALDEHYDE AND D-RIBOSE-5-PHOSPHATE USING AN ENZYMATIC SYSTEM, Journal of fermentation and bioengineering, 85(5), 1998, pp. 536-538
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
ISSN journal
0922-338X
Volume
85
Issue
5
Year of publication
1998
Pages
536 - 538
Database
ISI
SICI code
0922-338X(1998)85:5<536:PODF[A>2.0.ZU;2-V
Abstract
D-[1-C-13]Glucose is useful for studies on tracing by means of C-13-ma gnetic resonance imaging (IL.IRI) the fate of a metabolite in the brai n, and this technique is expected to become a sophisticated clinical t ool for the diagnosis of neurological disorders. For this purpose, a m ore efficient method of producing inexpensive C-13-labeled D-glucose i s necessary, and was investigated. When a mixture of the labeled D-fru ctose-6-phosphate (F-6-P) and D-glucose-6-phosphate (G-6-P) prepared f rom D-ribose-5-phosphate and [C-13]formaldehyde is dephosphorylated by potato acid phosphatase, a combination of potato acid phosphatase, ph osphoglucose isomerase, and BaCl2 was found to be effective in improvi ng the yield of D-glucose and shortening the reaction time. The maximu m yield of labeled D-glucose from the mixture of labeled F-6-P and G-6 -P was 84%. Two peaks indicative of labeled D-glucose with C-13 incorp orated at the C-l position were confirmed by the NMR spectroscopy.