CLONING AND SEQUENCING OF A GENE-CLUSTER FOR THE META-CLEAVAGE PATHWAY OF ANILINE DEGRADATION IN ACINETOBACTER SP. STRAIN YAA

Citation
M. Takeo et al., CLONING AND SEQUENCING OF A GENE-CLUSTER FOR THE META-CLEAVAGE PATHWAY OF ANILINE DEGRADATION IN ACINETOBACTER SP. STRAIN YAA, Journal of fermentation and bioengineering, 85(5), 1998, pp. 514-517
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
ISSN journal
0922-338X
Volume
85
Issue
5
Year of publication
1998
Pages
514 - 517
Database
ISI
SICI code
0922-338X(1998)85:5<514:CASOAG>2.0.ZU;2-O
Abstract
A 17.0-kb SacI fragment, which contains a large region just downstream of the aniline dioxygenase genes (atdA1-A5) of Acinetobacter sp. stra in YAA, was cloned from YAA plasmid DNA. We determined the nucleotide sequence of a 10.5-kb segment within the fragment, in which eleven gen es were found. Eight of the genes, designated atdSBCDEFGH, were homolo gous to the meta-cleavage pathway genes dmpQBCDEFGH and xylTEGFJQKI th e products of which are involved in the phenol and toluene degradation pathways, respectively. The GS-C content of the atdSBCDEFGH genes ran ges from 51.1% to 59.5% and is unusual for Acinetobacter genes (38-47% ), indicating that the genes originated from other bacteria. In additi on, these values are quite different from those of the upstream atdA1- A5 genes (40.1-47.7%). This result suggests that this aniline degradat ion gene cluster is a hybrid structure.