RHAMNAN SULFATE FROM CELL-WALLS OF MONOSTROMA-LATISSIMUM

Citation
Jb. Lee et al., RHAMNAN SULFATE FROM CELL-WALLS OF MONOSTROMA-LATISSIMUM, Phytochemistry, 48(6), 1998, pp. 921-925
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Plant Sciences
Journal title
ISSN journal
0031-9422
Volume
48
Issue
6
Year of publication
1998
Pages
921 - 925
Database
ISI
SICI code
0031-9422(1998)48:6<921:RSFCOM>2.0.ZU;2-Z
Abstract
Rhamnan sulfate from cell walls of Monostroma latissimum was purified through subsequent chromatographic systems. The purified polysaccharid e consisted of large amounts of rhamnose residues and appeared to be a n entire homopolysaccharide. Antithrombin activity was lower than rham nan sulfate previously obtained from M. nitidum but similar to standar d heparin. Studies of the major structural parts of the rhamnan sulfat e by periodate oxidation, Smith degradation and permethylation, showed it to consist of 1,3- and 1,2 linked rhamnose residues in a ratio of 3:2. Sulfate was mainly substituted at C-3 or C-4 in the 1,2-linked rh amnose residue. The detection of oligosaccharides by matrix-assisted l aser desorption/ionization time-of-flight mass spectrometry supports t his structure. These results suggested that rhamnan sulfate from the M onostromaceae has quite different structural properties from ulvan fro m the Ulvaceae. (C) 1998 Elsevier Science Ltd. All rights reserved.