CLASSIFICATION OF LACTOBACILLUS-HELVETICUS STRAINS BY IMMUNOLOGICAL DIFFERENCES IN EXTRACELLULAR PROTEINASES

Citation
N. Yamamoto et al., CLASSIFICATION OF LACTOBACILLUS-HELVETICUS STRAINS BY IMMUNOLOGICAL DIFFERENCES IN EXTRACELLULAR PROTEINASES, Bioscience, biotechnology, and biochemistry, 62(6), 1998, pp. 1228-1230
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
ISSN journal
0916-8451
Volume
62
Issue
6
Year of publication
1998
Pages
1228 - 1230
Database
ISI
SICI code
0916-8451(1998)62:6<1228:COLSBI>2.0.ZU;2-0
Abstract
Polyclonal antibodies against an extracellular proteinase of Lactobaci llus helveticus CP53 were raised. The antibodies reacted with a 170-kD a enzyme with activity and a 53-kDa protein that seemed to be a degrad ation product of the 170-kDa proteinase from results of immunoblotting ;. The antibodies reacted also with a 45-kDa extracellular proteinase of L. helveticus CP790. However, monoclonal antibodies to the CP790 pr oteinase did not react with the proteinase of L, helveticus CP53. Seve nteen strains of L. helveticus were tested for immunological reactivit y with the two kinds of antibodies. The strains all had the same react ivity as either strain CP53 or strain CP790. Eleven strains with the 4 5-kDa proteinase were identified as L. helveticus biovar jugurti becau se they did not ferment maltose, four other strains with the 170- and 53-kDa proteins were identified as L. helveticus biovar helveticus bec ause they fermented maltose, The remaining two strains dit not fit thi s pattern; they had both the 170- and 53-kDa proteins, but classificat ion by their sugar utilization showed them to be L. helveticus biovar jugurti.