ISOLATION OF A HIGHLY METASTATIC CELL-LINE TO LYMPH-NODE IN HUMAN ORAL SQUAMOUS-CELL CARCINOMA BY ORTHOTOPIC IMPLANTATION IN NUDE-MICE

Citation
T. Matsui et al., ISOLATION OF A HIGHLY METASTATIC CELL-LINE TO LYMPH-NODE IN HUMAN ORAL SQUAMOUS-CELL CARCINOMA BY ORTHOTOPIC IMPLANTATION IN NUDE-MICE, Oral Oncology, 34(4), 1998, pp. 253-256
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Dentistry,Oral Surgery & Medicine
Journal title
ISSN journal
1368-8375
Volume
34
Issue
4
Year of publication
1998
Pages
253 - 256
Database
ISI
SICI code
1368-8375(1998)34:4<253:IOAHMC>2.0.ZU;2-E
Abstract
To isolate a variant cell line with increased lymph node metastasising potential from human oral squamous cell carcinoma (SCC) we performed in vivo selection using orthotopic implantation in nude mice. Human or al SCC cells (HSC-3) were injected orthotopically into the tongue of n ude mice. After 3 weeks their cervical lymph nodes were excised and th e tumour cells metastasised to the lymph nodes were isolated and injec ted into the tongue of nude mice again, this procedure was performed t hree times. The resultant cells, designated as HSC-3-M3, metastasised to cervical lymph nodes in 90% of mice, while parental HSC-3 cells met astasised in only 30% of mice after injection into the tongue. HSC-3 a nd HSC-3-M3 cell lines which have the same origin but different lympha tic metastatic capacities could be a useful model system for studying mechanisms involved in lymph node metastasis of human oral SCC. (C) 19 98 Elsevier Science Ltd. All rights reserved.