T. Matsui et al., ISOLATION OF A HIGHLY METASTATIC CELL-LINE TO LYMPH-NODE IN HUMAN ORAL SQUAMOUS-CELL CARCINOMA BY ORTHOTOPIC IMPLANTATION IN NUDE-MICE, Oral Oncology, 34(4), 1998, pp. 253-256
To isolate a variant cell line with increased lymph node metastasising
potential from human oral squamous cell carcinoma (SCC) we performed
in vivo selection using orthotopic implantation in nude mice. Human or
al SCC cells (HSC-3) were injected orthotopically into the tongue of n
ude mice. After 3 weeks their cervical lymph nodes were excised and th
e tumour cells metastasised to the lymph nodes were isolated and injec
ted into the tongue of nude mice again, this procedure was performed t
hree times. The resultant cells, designated as HSC-3-M3, metastasised
to cervical lymph nodes in 90% of mice, while parental HSC-3 cells met
astasised in only 30% of mice after injection into the tongue. HSC-3 a
nd HSC-3-M3 cell lines which have the same origin but different lympha
tic metastatic capacities could be a useful model system for studying
mechanisms involved in lymph node metastasis of human oral SCC. (C) 19
98 Elsevier Science Ltd. All rights reserved.