GENE-TRANSFER AND PLANT-REGENERATION TECHNIQUES

Citation
R. Walden et R. Wingender, GENE-TRANSFER AND PLANT-REGENERATION TECHNIQUES, Trends in biotechnology, 13(9), 1995, pp. 324-331
Citations number
54
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
0167-7799
Volume
13
Issue
9
Year of publication
1995
Pages
324 - 331
Database
ISI
SICI code
0167-7799(1995)13:9<324:GAPT>2.0.ZU;2-T
Abstract
The production of transgenic plants depends on the stable introduction of foreign DNA into the plant genome, followed by regeneration to pro duce intact plants, and the subsequent expression of the introduced ge ne(s). A Variety of transformation methods have been developed, and th is has allowed many of the world's important crop plants to be transfo rmed. Transformation mediated by Agrobacterium has provided a reliable means of creating transgenics in a wide variety of species that are a menable to tissue culture and regeneration. Vectorless gene-transfer s ystems include particle bombardment (biolistics), electroporation and membrane permeabilization using chemicals. Of these, biolistics has pr oved to be particularly successful with plants that are less amenable to tissue culture, such as cereals and legumes.