ISOLATION AND ANALYSIS OF A CDNA CLONE ENCODING THE SMALL-SUBUNIT OF ADP-GLUCOSE PYROPHOSPHORYLASE FROM WHEAT

Citation
C. Ainsworth et al., ISOLATION AND ANALYSIS OF A CDNA CLONE ENCODING THE SMALL-SUBUNIT OF ADP-GLUCOSE PYROPHOSPHORYLASE FROM WHEAT, Plant molecular biology, 23(1), 1993, pp. 23-33
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences",Biology
Journal title
ISSN journal
0167-4412
Volume
23
Issue
1
Year of publication
1993
Pages
23 - 33
Database
ISI
SICI code
0167-4412(1993)23:1<23:IAAOAC>2.0.ZU;2-W
Abstract
A full-length cDNA clone from hexaploid bread wheat, encoding the smal l subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame whic h encodes a protein of 473 amino acids (52.1 kDa). The presence of a c hloroplast/amyloplast transit peptide of 22 amino acids is proposed. T he deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (w ith 90% of identical amino acids) and potato (with 86% of identical am ino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.