EXPRESSION PATTERN OF SYNUCLEINS (NON-A-BETA COMPONENT OF ALZHEIMERS-DISEASE AMYLOID PRECURSOR PROTEIN ALPHA-SYNUCLEIN) DURING MURINE BRAIN-DEVELOPMENT

Citation
Lj. Hsu et al., EXPRESSION PATTERN OF SYNUCLEINS (NON-A-BETA COMPONENT OF ALZHEIMERS-DISEASE AMYLOID PRECURSOR PROTEIN ALPHA-SYNUCLEIN) DURING MURINE BRAIN-DEVELOPMENT, Journal of neurochemistry, 71(1), 1998, pp. 338-344
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Neurosciences
Journal title
ISSN journal
0022-3042
Volume
71
Issue
1
Year of publication
1998
Pages
338 - 344
Database
ISI
SICI code
0022-3042(1998)71:1<338:EPOS(C>2.0.ZU;2-V
Abstract
The non-A beta component of Alzheimer's disease amyloid precursor prot ein (NACP) is predominantly a neuron-specific presynaptic protein that may play a central role in neurodegeneration because NACP fragments a re found in Alzheimer's disease amyloid and a mutation in the NACP gen e is associated with familiar Parkinson's disease, In addition, NACP m ay play an important role during synaptogenesis and CNS development. T o understand better the patterns of NACP expression during development , we analyzed the levels of this protein as well as the levels of anot her synaptic protein (synaptophysin) by ribonuclease protection assay, western blotting, and immunocytochemistry in fetal, juvenile, and adu lt mouse brain. From embryonic day 12 to 15, there was a slight increa se, which was then followed by a more dramatic increase at later time points. Immunocytochemical staining for NACP increases throughout thes e stages as well. Although NACP appeared early in CNS development, syn aptophysin levels started to rise at a later stage. These findings sup port the contention that NACP might be important for CNS development. Furthermore, the cytosolic component of NACP precedes the particulate component in development, indicating that a redistribution of the prot ein to the membrane fraction may be important for events later in neur onal development and in synaptogenesis.