MICRO-FABRICATION AND ANALYTICAL PERFORMA NCES OF QUARTZ AND GLASS MICROCHIPS FOR ELECTROPHORESIS

Citation
H. Nakanishi et al., MICRO-FABRICATION AND ANALYTICAL PERFORMA NCES OF QUARTZ AND GLASS MICROCHIPS FOR ELECTROPHORESIS, Bunseki Kagaku, 47(6), 1998, pp. 361-368
Citations number
3
Language
GIAPPONESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
0525-1931
Volume
47
Issue
6
Year of publication
1998
Pages
361 - 368
Database
ISI
SICI code
0525-1931(1998)47:6<361:MAAPNO>2.0.ZU;2-4
Abstract
Microchips for capillary electrophoresis have been fabricated in small size on quartz or glass substrates. Each microchip is 11 mm x 28 mm i n size and 1.5 mm in thickness. It has a sample loading channel, a sep aration channel and four reservoirs at the ends of each channel. To fa bricate a quartz chip, we applied a novel bonding method with a 1% (w/ v) hydrofluoric acid solution. Using fabricated microchips, we evaluat ed several of their analytical features. We have shown that the microc hips permit quantitative sample injection (several 10 pi or less) with an adequate potential control, high-speed analysis (30 s) due to the high heat-dissipation capability and effective separation (the number of theoretical plates is over 2500 at separation length of 17 mm).