CHROMOSOME ABERRATION ASSAYS IN CREPIS FOR THE STUDY OF ENVIRONMENTALMUTAGENS

Authors
Citation
Wf. Grant et Et. Owens, CHROMOSOME ABERRATION ASSAYS IN CREPIS FOR THE STUDY OF ENVIRONMENTALMUTAGENS, Mutation research-reviews in mutation research, 410(3), 1998, pp. 291-307
Citations number
189
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Genetics & Heredity",Toxicology,"Biothechnology & Applied Migrobiology
Volume
410
Issue
3
Year of publication
1998
Pages
291 - 307
Database
ISI
SICI code
Abstract
Crepis capillaris (2n = 6) is an excellent plant for the assay of chro mosome aberrations after chemical treatment. C. tectorum (2n = 8) has been used also in mutagenic studies, but to a much lesser extent. A pr otocol has been given for using root tips to study the cytological end points, such as chromosome breaks and exchanges, which follow the test ing of chemicals in somatic cells. Meiotic endpoints have not been use d in C. capillaris for testing potential chemical mutagens but should be considered, especially a meiotic micronucleus assay. From a literat ure survey, 81 chemicals are tabulated that have been assayed in 162 C repis assays for their clastogenic effects. Of the 162 assays that hav e been carried out, 40 are reported at giving a positive reaction (i.e ., causing chromosome aberrations), 97 positive and with a dose respon se, 7 borderline positive, and 17 negative. Eighty-five percent of the chemicals gave a definite positive response. Assays for one chemical gave contrary results, and were not included in the above tabulation. The Crepis bioassay has been shown to be an excellent plant bioassay f or assessing chromosome damage induced by chemicals and environmental pollutants. (C) 1998 Elsevier Science B.V. All rights reserved.