Stochastic and nonstochastic post-transcriptional silencing of chitinase and beta-1,3-glucanase genes involves increased RNA turnover - Possible rolefor ribosome-independent RNA degradation

Citation
H. Holtorf et al., Stochastic and nonstochastic post-transcriptional silencing of chitinase and beta-1,3-glucanase genes involves increased RNA turnover - Possible rolefor ribosome-independent RNA degradation, PL CELL, 11(3), 1999, pp. 471-483
Citations number
63
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL
ISSN journal
1040-4651 → ACNP
Volume
11
Issue
3
Year of publication
1999
Pages
471 - 483
Database
ISI
SICI code
1040-4651(199903)11:3<471:SANPSO>2.0.ZU;2-Y
Abstract
Stochastic and nonstochastic post-transcriptional gene silencing (PTGS) in Nicotiana sylvestris plants carrying tobacco class I chitinase (CHN) and be ta-1,3-glucanase transgenes differs in incidence, stability, and pattern of expression. Measurements with inhibitors of RNA synthesis (cordycepin, act inomycin D, and alpha-amanitin) showed that both forms of PTGS are associat ed with increased sequence-specific degradation of transcripts, suggesting that increased RNA turnover may be a general feature of PTGS. The protein s ynthesis inhibitors cycloheximide and verrucarin A did not inhibit degradat ion of CHN RNA targeted for PTGS, confirming that PTGS-related RNA degradat ion does not depend on ongoing protein synthesis. Because verrucarin A, unl ike cycloheximide, dissociates mRNA from ribosomes, our results also sugges t that ribosome-associated RNA degradation pathways may not be involved in CHN PTGS.