A NOVEL BIOMARKER FOR HYPERGLYCEMIA, MRX ISOLATED FROM HYDROLYSATE OFGLYCATED PROTEINS

OYA T KUMON H KOBAYASHI H AKIBA M HOSOKAWA T KAKIMOTO N NAKAMURA K MORIMITSU Y OSAWA T

T. Oya et al., A NOVEL BIOMARKER FOR HYPERGLYCEMIA, MRX ISOLATED FROM HYDROLYSATE OFGLYCATED PROTEINS, Biochemical and biophysical research communications, 246(1), 1998, pp. 267-271

25

INGLESE

Article

Biology,Biophysics

Biochemical and biophysical research communications → ACNP

0006-291X

246

1

1998

267 - 271

ISI

0006-291X(1998)246:1<267:ANBFHM>2.0.ZU;2-Q

Long-lived proteins can undergo non-enzymatic glycation to form highly crosslinked structures with characteristic fluorescence during aging and diabetes processes. In this paper, a typical fluorophore, named Ma illard reaction product X (MRX), was isolated from the hydrolysate of glycated proteins. MRX could be formed by incubation of bovine serum a lbumin with glucose, followed by acid hydrolysis. The structure of MRX was determined to be ihydrothiazolo[3,2-alpha]pyridinium-3-carboxylat e. MRX was also found to be formed by the incubation of cysteine and a rginine with glucose, followed by hydrolysis. We found the formation o f MRX in the recently developed genetically diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats and compared them with that in the contr ol Long-Evans Tokushima Otsuka (LETO) rats. Significantly higher level s of MRX were observed from the serum (p < 0.005) and urinary protein (p < 0.001) of OLETF rats in comparison with those of LETO rats. MRX m ust be a potential candidate as a biomarker for hyperglycemia. (C) 199 8 Academic Press.