IMMUNOCYTOCHEMICAL LOCALIZATION OF SALIVARY PEPTIDE P-C IN HUMAN SUBMANDIBULAR-GLAND

Citation
I. Kimura et al., IMMUNOCYTOCHEMICAL LOCALIZATION OF SALIVARY PEPTIDE P-C IN HUMAN SUBMANDIBULAR-GLAND, Biological & pharmaceutical bulletin, 21(5), 1998, pp. 506-512
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy
ISSN journal
0918-6158
Volume
21
Issue
5
Year of publication
1998
Pages
506 - 512
Database
ISI
SICI code
0918-6158(1998)21:5<506:ILOSPP>2.0.ZU;2-5
Abstract
Human saliva contains a proline-rich polypeptide, salivary peptide P-C , which potentiates insulin release and reduces glucagon release from perfused rat pancreas to decrease blood glucose level, To elucidate th e process of secretion into humoral fluid of this peptide morphologica lly we investigated ultrastructural localization of P-C in human subma ndibular gland by immunogold technique with anti-peptide P-C whose spe cificity to P-C mas confirmed bq. immunoblotting. The labeling with go ld particles which represents the distribution of P-C-like-immunoreact ivity (P-C-LI) mas detected in the secretory granules and rough endopl asmic reticula of the acinar serous cells and in few mucosa cells. P-C -LI was also observed in the lumen of striated duct but not intracellu larly in the ductal cells themselves, indicating that P-C is not proba bly reabsorbed there, These results suggest that salivary peptide P-C is present in acinar serous cells, is secreted into the oral cavity an d may be reabsorbed through the digestive tract to modulate the blood glucose level after feeding.