DIVERGENCE IN THE EXPRESSION OF MOLECULAR MARKERS OF NEURONAL ACTIVATION IN THE PARVOCELLULAR PARAVENTRICULAR NUCLEUS OF THE HYPOTHALAMUS EVOKED BY ALCOHOL ADMINISTRATION VIA DIFFERENT ROUTES

Citation
Km. Ogilvie et al., DIVERGENCE IN THE EXPRESSION OF MOLECULAR MARKERS OF NEURONAL ACTIVATION IN THE PARVOCELLULAR PARAVENTRICULAR NUCLEUS OF THE HYPOTHALAMUS EVOKED BY ALCOHOL ADMINISTRATION VIA DIFFERENT ROUTES, The Journal of neuroscience, 18(11), 1998, pp. 4344-4352
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0270-6474
Volume
18
Issue
11
Year of publication
1998
Pages
4344 - 4352
Database
ISI
SICI code
0270-6474(1998)18:11<4344:DITEOM>2.0.ZU;2-1
Abstract
Immediate early gene (IEG) expression has been routinely used by neuro scientists as an index of neuronal activation. In the case of the hypo thalamic-pituitary-adrenal axis, induction of c-fos and/or NGFI-B mRNA s in the parvocellular paraventricular nucleus (pPVN) has been documen ted after a variety of stimuli that increase adrenocorticotropin (ACTH ) in the systemic circulation. However, the functional relationship be tween expression of IEGs and transcription of the genes for the ACTH s ecretagogues corticotropin-releasing factor (CRF) and arginine vasopre ssin (AVP) is not clear. While investigating the neuroendocrine correl ates of alcohol administration via different routes (intraperitoneal v s intragastric), we noted a difference in the time course of NGFI-B mR NA expression in the pPVN, despite comparable dynamics in ACTH secreti on. By comparing the temporal cascade of transcriptional events in viv o after alcohol injection via either route, we sought to determine fun ctional relationships between IEGs and the induction of GRF and AVP he teronuclear RNAs (hnRNAs). One advantage of our paradigm is the use of the same stimulus (systemic alcohol injection) in which access to the CNS does not differ between the groups to be compared. Intraperitonea l administration of the drug resulted in significant increases in c-fo s mRNA, Fos protein, CRF hnRNA, and AVP hnRNA. in contrast, intragastr ic treatment evoked a brief, modest elevation in c-fos mRNA and Fos pr otein, increased AVP hnRNA, and caused no detectable change in CRF hnR NA. These data indicate that robust increases in CRF hnRNA are closely linked to full expression of c-fos mRNA and Fos protein. In addition, the expression of NGFI-B after both routes of administration is indic ative of cellular activation within the pPVN in parallel with secretio n of ACTH.