OPTIMIZATION OF THE ENUMERATION OF TOTAL AEROBIC BACTERIAL-FLORA IN THE FLESH OF SEAFISH

Citation
M. Valle et al., OPTIMIZATION OF THE ENUMERATION OF TOTAL AEROBIC BACTERIAL-FLORA IN THE FLESH OF SEAFISH, Journal of rapid methods and automation in microbiology, 6(1), 1998, pp. 29-42
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science & Tenology
ISSN journal
1060-3999
Volume
6
Issue
1
Year of publication
1998
Pages
29 - 42
Database
ISI
SICI code
1060-3999(1998)6:1<29:OOTEOT>2.0.ZU;2-W
Abstract
The total aerobic flora of seafish flesh is weakly halophilic, and req uires on average 1.38% NaCl according to statistical studies. Enumerat ion is optimal on tryptone soya agar or on NaCl supplemented plate cou nt agar (-H2S), incubated at 20 and 25C, respectively. Plate count aga r(-H2S) was selected because it can also be used for enumeration of hy drogen sulfide-producing bacteria by degradation of sulfur-containing proteins, which are abundant in fish The models employed are sigmoidal . The initial bioburden is too great for there to be a lag phase durin g storage in ice at 0C. The models show that when the total aerobic mi croflora count exceeds 100, 000 cfu/g, whole or filleted fish stored o n ice at 0C are unfit for consumption.