FLUOROMETRIC-DETERMINATION OF EPINEPHRINE WITH O-PHENYLENEDIAMINE

Citation
Jh. Yang et al., FLUOROMETRIC-DETERMINATION OF EPINEPHRINE WITH O-PHENYLENEDIAMINE, Analytica chimica acta, 363(1), 1998, pp. 105-110
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical
Journal title
Volume
363
Issue
1
Year of publication
1998
Pages
105 - 110
Database
ISI
SICI code
Abstract
A simple and sensitive fluorimetric method for the determination of ep inephrine is described. The method is based on the reaction of epineph rine with o-phenylenediamine in alkaline medium. The addition of 0.2% (v/v) acetone enhances the fluorescence 50-fold. Under optimum conditi ons, a linear relationship was obtained between the fluorescence inten sity and the concentration of epinephrine in the 2.0 x 10(-8)similar t o 6.0 x 10(-6) mol/l range; the detection limit is 9.3 x 10(-9) mol/l. In recovery tests, the relative standard deviation (five replicates) was within 2.0% over the linear range. (C) 1998 Elsevier Science B.V.