TACTILE HYPERESTHESIA, ALTERED EPIDERMAL INNERVATION AND PLANTAR NERVE INJURY IN THE HINDFEET OF RATS HOUSED ON WIRE GRATES

Citation
Ap. Mizisin et al., TACTILE HYPERESTHESIA, ALTERED EPIDERMAL INNERVATION AND PLANTAR NERVE INJURY IN THE HINDFEET OF RATS HOUSED ON WIRE GRATES, Brain research, 788(1-2), 1998, pp. 13-19
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
Volume
788
Issue
1-2
Year of publication
1998
Pages
13 - 19
Database
ISI
SICI code
Abstract
The effects of wire grates on nerve injury and recovery were examined in rats housed in cages with sawdust-covered solid flooring. For the f irst 3 weeks of the study, 20 rats were housed on sawdust alone and 20 rats were housed in cages with wire grates placed over the sawdust. F or the remaining 9 weeks, 10 animals housed on sawdust had wire grates added to their cages, while grates were removed from the cages of 10 animals. The effects of tactile stimulation on hindpaw plantar skin wa s measured weekly using the Von Frey filament test. Intraepidermal inn ervation using PGP 9.5 immunostaining and plantar nerve histology were assessed at the end of the 12-week study. After just 1 week on grates , hindpaw withdrawal thresholds were already markedly decreased and re mained low until the grates were removed at 3 weeks. Thresholds return ed to normal by 4 weeks after removal of the grates. Wire grates also induced increases in PGP 9.5 immunoreactive intraepidermal fine nerve endings that were normalized after grate removal. Demyelination, Walle rian degeneration and Renaut bodies were induced in the medial plantar nerve in rats housed in cages with wire-grate flooring. Nerve injury was largely resolved after 9 weeks on sawdust flooring. These data dem onstrate that wire grates rapidly induce hindpaw tactile hyperesthesia and plantar neuropathy in rats and emphasize a risk of using wire-gra te cage flooring in studies assessing hindlimb function and structure. (C) 1998 Elsevier Science B.V.