ROLE FOR THE VACCINIA VIRUS A36R OUTER ENVELOPE PROTEIN IN THE FORMATION OF VIRUS-TIPPED ACTIN-CONTAINING MICROVILLI AND CELL-TO-CELL VIRUSSPREAD

Citation
Ej. Wolffe et al., ROLE FOR THE VACCINIA VIRUS A36R OUTER ENVELOPE PROTEIN IN THE FORMATION OF VIRUS-TIPPED ACTIN-CONTAINING MICROVILLI AND CELL-TO-CELL VIRUSSPREAD, Virology, 244(1), 1998, pp. 20-26
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology
Journal title
ISSN journal
0042-6822
Volume
244
Issue
1
Year of publication
1998
Pages
20 - 26
Database
ISI
SICI code
0042-6822(1998)244:1<20:RFTVVA>2.0.ZU;2-3
Abstract
A small-plaque-forming vaccinia virus mutant with a deletion in the A3 6R gene encoding an outer envelope protein (Parkinson and Smith, Virol ogy, 204, 376-390, 1994) was shown to assemble wrapped forms of intra- and extracellular virus particles and to mediate acid-induced polykary on formation. The intracellular virions, however, did not acquire acti n tails and those on the cell surface were not associated with special ized microvilli. This phenotype is similar to that of the A34R (E. J. Wolffe, E. Katz, A. Weisberg, and a. Moss, J. Virol. 71, 3904-3915, 19 97) and A33R (R. Roper, E.J. Wolffe, A Weisberg, and a. Moss, J. Virol ., in press) deletion mutants. Taken together, these data support a mo del in which the envelope proteins encoded by the A33R, A34R, and A36R genes are all required for nucleation of actin tails, which facilitat e dissemination rather than egress of virus particles.