T. Masuda et al., HOW THICK ARE THE PARAFFIN-EMBEDDED TISSUE-SECTIONS ROUTINELY PREPARED IN LABORATORY - A MORPHOMETRIC STUDY USING A CONFOCAL LASER-SCANNINGMICROSCOPE, Pathology international, 48(3), 1998, pp. 179-183
In order to evaluate the differences between cut and measured thicknes
ses of formalin-fixed, paraffin-embedded tissue sections, formalin-fix
ed, paraffin-embedded blocks of liver tissue were prepared from four m
ale Wistar rats, The sections were cut at preselected thicknesses of 3
, 6, 9, 12, 15 and 18 mu m, and observed under confocal laser scanning
microscopy. The maximum and minimum section thicknesses were measured
using a vertically cut section image constructed by the software inco
rporated in the microscope, The surface of the section was not smooth
but showed fine undulations in addition to a slight inclination, The m
ean and range of the section thicknesses measured were 4.3 +/- 1.3, 7.
0 +/- 1.7, 9.8 +/- 2.1, 12.0 +/- 2.6, 14.1 +/- 3.0, and 17.7 +/- 3.8 m
u m for the preselected cut thicknesses of 3, 6, 9, 12, 15 and 18 mu m
. It is stressed that the evaluated parameters of the section (volume
or surface densities) include standard errors of up to 10% caused by t
he differences between the cut and real thicknesses.