SENSITIVITY OF KAPOSIS-SARCOMA-ASSOCIATED HERPESVIRUS REPLICATION TO ANTIVIRAL DRUGS - IMPLICATIONS FOR POTENTIAL THERAPY

Authors
Citation
Dh. Kedes et D. Ganem, SENSITIVITY OF KAPOSIS-SARCOMA-ASSOCIATED HERPESVIRUS REPLICATION TO ANTIVIRAL DRUGS - IMPLICATIONS FOR POTENTIAL THERAPY, The Journal of clinical investigation, 99(9), 1997, pp. 2082-2086
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
0021-9738
Volume
99
Issue
9
Year of publication
1997
Pages
2082 - 2086
Database
ISI
SICI code
0021-9738(1997)99:9<2082:SOKHRT>2.0.ZU;2-H
Abstract
Using a cell line (termed BCBL-1) derived from a peripheral effusion ( body cavity-based) lymphoma latently infected with Kaposi's sarcoma-as sociated herpesvirus (KSHV), we recently reported the successful induc tion of KSHV replication in culture (Renne, R., W. Zhong, B. Herndier, M. McGrath, N. Abbey, D. Kedes, and D. Ganem. 1996, Nat. Med. 2:342-3 46). Here we report the first use of this system for establishing the susceptibility of KSHV to available antiviral drugs. Latently infected BCBL-1 cells were induced to lytic replication with phorbol esters; s uch cells secrete large numbers of KSHV virions into the culture mediu m, We assayed the ability of the antivirals to block KSHV production, as measured by the release of encapsidated viral DNA, The results show that KSHV replication is insensitive to acyclovir (9-[(2-hydroxyethox y)-methyl]guanine) (50% inhibitory concentration [IC50] = 60-80 mu M), but sensitive to ganciclovir (9-[1,3-dihydroxy-2-propoxymethyl]guanin e) (IC50 = 2.7-4 mu M), foscarnet (trisodium phosphonoformate hexahydr ate) (IC50 = 80-100 mu M), and cidofovir (S)-3-hydroxy-2-(phosphonomet hoxy)propyl]cytosine) (IC50 = 0.5-1 mu M).