BRAIN-DERIVED NEUROTROPHIC FACTOR MODULATES THE DEVELOPMENT OF THE DOPAMINERGIC NETWORK IN THE RODENT RETINA

Citation
A. Cellerino et al., BRAIN-DERIVED NEUROTROPHIC FACTOR MODULATES THE DEVELOPMENT OF THE DOPAMINERGIC NETWORK IN THE RODENT RETINA, The Journal of neuroscience, 18(9), 1998, pp. 3351-3362
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0270-6474
Volume
18
Issue
9
Year of publication
1998
Pages
3351 - 3362
Database
ISI
SICI code
0270-6474(1998)18:9<3351:BNFMTD>2.0.ZU;2-H
Abstract
Dopaminergic cells in the retina express the receptor for brain-derive d neurotrophic factor (BDNF) (Cellerino and Kohler, 1997). To investig ate whether BDNF can influence the development of the retinal dopamine rgic pathway, we performed intraocular injections of BDNF during the s econd or third postnatal week and visualized the dopaminergic system w ith tyrosine hydroxylase (TH) immunohistochemistry. Both regimens of B DNF treatment caused an increase in TH immunoreactivity in stratum 1 a nd stratum 3 of the inner plexiform layer ([PL). D2 dopamine receptor immunoreactivity, a presynaptic marker of dopaminergic cells (Veruki, 1996), was also increased in stratum 1 and stratum 3 of the inner plex iform layer. These data suggest that BDNF causes sprouting of dopamine rgic fibers in the inner plexiform layer. Other neurochemical systems, for example, the cholinergic amacrine cells, remained unaffected. Sim ilar effects were observed after injections of neurotrophin-3 and neur otrophin-4, but not nerve growth factor. Analysis of whole-mounted TH- immunolabeled retinae revealed hypertrophy of dopaminergic cells(+41% in soma areas; p < 0.01) and an increase of labeled dopaminergic varic osities in stratum 1 of the IPL (+51 %; p < 0.01) after BDNF treatment . The opposite was observed in mice homozygous for a null mutation of the bdnf gene: dopaminergic cells were atrophic (-22.5% in soma areas; p < 0.05), and the density of TH-positive varicosities in stratum 1 w as reduced (57%; p < 0.01). We conclude that BDNF controls the develop ment of the retinal dopaminergic network and may be particularly impor tant in determining the density of dopaminergic innervation in the ret ina.