OPEN READING FRAME L1 OF MAREKS-DISEASE HERPESVIRUS IS NOT ESSENTIAL FOR IN-VITRO AND IN-VIVO VIRUS-REPLICATION AND ESTABLISHMENT OF LATENCY

Citation
Ka. Schat et al., OPEN READING FRAME L1 OF MAREKS-DISEASE HERPESVIRUS IS NOT ESSENTIAL FOR IN-VITRO AND IN-VIVO VIRUS-REPLICATION AND ESTABLISHMENT OF LATENCY, Journal of General Virology, 79, 1998, pp. 841-849
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology,"Biothechnology & Applied Migrobiology
Journal title
ISSN journal
0022-1317
Volume
79
Year of publication
1998
Part
4
Pages
841 - 849
Database
ISI
SICI code
0022-1317(1998)79:<841:ORFLOM>2.0.ZU;2-K
Abstract
Two mutant CV1988 Marek's disease virus (MDV) strains were developed i n which a part of ORF L1 was replaced by lacZ with the SV40 early prom oter. These mutant strains, CVIL(1)LacZ-A and -B, were inoculated into chickens to test the hypothesis that ORF L1 is involved in the induct ion and/or maintenance of latency. Mutant virus could be reisolated fr om lymphocytes obtained from chickens during both the lytic and latent phase of infection, indicating that ORF L1 is not essential for the i nduction and/or maintenance of latency or the reactivation from latenc y. beta-Galactosidase-positive lymphocytes were detected during the la tent infection demonstrating that the SV40 early promoter can be activ e in recombinant MDV strains during latent infection, Although the ins ertion of lacZ was stable in cell culture, recombination within lacZ a nd the BamHI-L fragment was observed during in vivo infection.