GENE-TRANSFER AND KIDNEY-DISEASE

Citation
E. Imai et al., GENE-TRANSFER AND KIDNEY-DISEASE, JN. Journal of nephrology, 11(1), 1998, pp. 16-19
Citations number
27
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Urology & Nephrology
Journal title
ISSN journal
1121-8428
Volume
11
Issue
1
Year of publication
1998
Pages
16 - 19
Database
ISI
SICI code
1121-8428(1998)11:1<16:GAK>2.0.ZU;2-V
Abstract
There is little doubt that molecular biological intervention therapy h as come of age and its potential is arousing tremendous excitement. A gene transfer technique, the HVJ-liposome method, is now applicable as a tool for the dissection of molecular aspects in the pathophysiology of renal diseases, and for gene therapy in experimental glomeruloneph ritis. Overexpressed transforming growth factor (TGF)-beta in the norm al rat glomeruli, by gene transfer of TGF-beta cDNA, leads to glomerul osclerosis. However, inhibition of the TGF-beta action by antisense ol igonucleotides can suppress the development of the experimental glomer ulonephritis. We investigated whether in vivo gene transfer of chimeri c proteins, composed of the extracellular domain of TGF-beta type II r eceptor fused with IgC-Fc, suppresses experimental glomerulonephritis. The expression of TGF-beta in glomeruli was suppressed and so was the extracellular matrix expansion. Taken together with clinical observat ion of up-regulation of TGF-beta in various glomerulopathies, the dys- regulation of the TGF-beta is important in the development of glomerul osclerosis, and manipulation of this overexpression may prove a novel therapeutic approach for slowing the progression of the disease.