COUPLING MATRIX-ASSISTED-LASER-DESORPTION IONIZATION TO LIQUID SEPARATIONS/

Authors
Citation
Kk. Murray, COUPLING MATRIX-ASSISTED-LASER-DESORPTION IONIZATION TO LIQUID SEPARATIONS/, Mass spectrometry reviews, 16(5), 1997, pp. 283-299
Citations number
103
Language
INGLESE
art.tipo
Review
Journal title
ISSN journal
0277-7037
Volume
16
Issue
5
Year of publication
1997
Pages
283 - 299
Database
ISI
SICI code
0277-7037(1997)16:5<283:CMITLS>2.0.ZU;2-C
Abstract
This review discusses the off-line and art-line coupling of matrix-ass isted laser desorption/ionization (MALDI) mass spectrometry to liquid chromatography (LC) and capillary electrophoresis (CE). Off-line metho ds involve collecting fractions or depositing a sample on a prepared t arget prior to MALDI analysis. Because LC fraction collection is strai ghtforward off-line MALDI LC-MS is relatively easy to implement and wi dely used. Collecting CE fractions poses a greater challenge due to th e small sample volume and the high voltage electrical connection that must be maintained during separation. Methods for off-line MALDI CE-MS employing a porous glass joint, liquid sheath flow, low-volume buffer or direct deposition are discussed. On-line coupling of MALDI to liqu id separations involves introducing the sample directly into the mass spectrometer, using either a continuous-flow (CF) probe or an aerosol spray. With CF MALDI, liquid samples are delivered to the probe throug h a silica capillary. Aerosol MALDI relies on a pneumatic nebulizer to deliver samples to the mass spectrometer, where ions are formed throu gh irradiation of the particles. CF MALDI and aerosol MALDI have both been coupled to LC separations, although further refinements in both t echniques are needed. Future prospects for liquid separations and both off-line and on-line MALDI are discussed. (C) 1998 John Wiley & Sons, Inc.