REACTIVE IRON SPECIES IN BIOLOGICAL-FLUIDS ACTIVATE THE IRON-SULFUR CLUSTER OF ACONITASE

Citation
S. Mumby et al., REACTIVE IRON SPECIES IN BIOLOGICAL-FLUIDS ACTIVATE THE IRON-SULFUR CLUSTER OF ACONITASE, Biochimica et biophysica acta (G). General subjects, 1380(1), 1998, pp. 102-108
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics
ISSN journal
0304-4165
Volume
1380
Issue
1
Year of publication
1998
Pages
102 - 108
Database
ISI
SICI code
0304-4165(1998)1380:1<102:RISIBA>2.0.ZU;2-5
Abstract
Low molecular mass iron (LMrFe) can appear in plasma when the transfer rin becomes fully iron loaded. Such iron poses a risk factor for oxida tive damage, and for microbial virulence. A previous novel approach to the detection and measurement of LMrFe in plasma was the use of the i ron-binding properties of the glycopeptide antitumour antibiotic bleom ycin and its ability to degrade DNA In the presence of oxygen, bound i ron, and an iron reducing agent. Since bleomycin is a non-physiologica l , ligand with iron-binding and redox cycling properties, it has been suggested that it may not be a valid biological model far detecting a nd measuring LMrFe. To address these concerns we have developed a biol ogical approach to the detection and measurement of LMrFe based on the activation of iron-requiring aconitase., Parallel measurements, in a variety of clinical conditions in which there was a complete saturatio n of the plasma transferrin, showed that the bleomycin assay and the a conitase assay can give similar results for LMrFe. (C) 1998 Elsevier S cience B.V.