IN-VITRO METABOLISM OF PHENYLALANINE BY RUMINAL BACTERIA, PROTOZOA, AND THEIR MIXTURE

Authors
Citation
Mr. Amin et R. Onodera, IN-VITRO METABOLISM OF PHENYLALANINE BY RUMINAL BACTERIA, PROTOZOA, AND THEIR MIXTURE, Journal of General and Applied Microbiology, 43(1), 1997, pp. 1-7
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0022-1260
Volume
43
Issue
1
Year of publication
1997
Pages
1 - 7
Database
ISI
SICI code
0022-1260(1997)43:1<1:IMOPBR>2.0.ZU;2-H
Abstract
An in vitro study was conducted to examine the metabolism of phenylala nine (Phe) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP), Rumen microorganisms were collected fro m fistulated goats fed lucerne cubes (Medicago sativa) and a concentra ted mixture twice a day. Microbial suspensions were anaerobically incu bated at 39 degrees C for 12 h, Phe and some other related compounds i n both supernatants and microbial hydrolysates of the incubations were analysed by HPLC. The net degradation rate (mu mol/g microbial nitrog en) of Phe in a was about 1.5-fold higher than that in P. Phe was conv erted mainly into phenylacetic acid (PAA) and unknown compound(s) that presumably involved tyrosine in B, P, and BP during the 12h incubatio n period, Small amounts of benzoic acid (BZA), and traces of phenylpro pionic acid (PPR) and phenyllactic acid (PLA) were also produced from Phe. PAA production in a was found to be higher than that in P,whereas it was significantly higher in BP. Although BZA production was less t han one-tenth that of PAA production, it was higher in P than in B and BP, PPR was detected in both B and BP, but not in P, PLA was detected only in B. The production of unknown compound(s) was higher in a than in P and BP.